Protein A-Agarose from Santa Cruz Biotechnology, Inc.

Product Specs
ItemProtein A-Agarose
CompanySanta Cruz Biotechnology, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Numbersc-2001
Quantity2 ml
MatrixAgarose
TypeAgarose

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) USP39 interacts with SIRT7 to promote cervical squamous cell carcinoma by modulating autophagy and oxidative stress via FOXM1Journal of Translational MedicineNovember 13, 2023Juanpeng Yu, Shuai Yuan, Jinglin Song, Shengsheng YuImmunoprecipitation was performed with Flag-USP39 and Myc-SIRT7. After culturing with protein-A agarose (sc-2001, Santa Cruz), the proteins were separated by 10% SurePAGE. The binding between USP39 and SIRT7 was evaluated

(2) CCT8 promotes cell migration and tumor metastasis in lung adenocarcinomasJournal of CancerOctober 2, 2023Zhiqiang Wu, Liyuan Deng, Jin Tao, Yuhai Lu, Xiaofei Zeng, Weikun Jia, Hu Chennormal rabbit IgG (2729, CST, 1:100) on a rotator overnight at 4 ℃, followed by addition of protein A (sc-2001, Santa Cruz) agarose beads and incubation for a further 2h at 4 ℃. After four washes in PBST, samples

(3) Lnc‑RGS5 sponges miR‑542‑5p to promote FoxM1/VEGFA signaling and breast cancer cell proliferationInternational journal of oncologyOctober 1, 2023Song J, Tang Y, Song F.ProteinTech Group, Inc.) antibodies at 4°C overnight. A total of 30 μl Protein A-Agarose beads (cat. no. sc-2001; Santa Cruz Biotechnology, Inc.) were added and incubated at 4°C for 2 h. After centrifugation at 200

(4) The histone chaperone function of Daxx is dispensable for embryonic developmentCell Death DisAugust 26, 2023Chang Sun, Yuan Qi, Natalie Fowlkes, Nina Lazic, Xiaoping Su, Guillermina Lozano, Amanda R. Wasylishenrotary agitation overnight. Cell lysates were then incubated with Protein A-coupled agarose beads (sc-2001, Santa Cruz Biotechnology, 20 μl) under rotary agitation for 4 h. The beads were washed using the buffer

(5) Ubiquitination of acetyltransferase Gcn5 contributes to fungal virulence in Fusarium graminearummBioJuly 28, 2023Ahai Chen, Yifan Zhou, Yiyi Ren, Chao Liu, Xingmin Han, Jing Wang, Zhonghua Ma, Yun Chenantibody (no. 39756, Active Motif). Immunoprecipitated DNA was enriched using protein A agarose beads (sc-2001; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and washed sequentially with low-salt wash buffer, high-salt

(6) The Phosphatase Cascade Nem1/Spo7-Pah1 Regulates Fungal Development, Lipid Homeostasis, and Virulence in Botryosphaeria dothideaMicrobiology SpectrumJune 15, 2023Weichao Ren, Yihan Zhang, Meiqi Zhu, Zequn Liu, Sen Lian, Caixia Wang, Baohua Li, Na Liu(Invitrogen-Thermo Fisher Scientific, MA, USA) as the mock sample. In addition, protein A agarose beads (sc-2001; Santa Cruz Biotechnology, Dallas, TX, USA) were added into IP and mock samples before incubation with

(7) FOXK2 amplification and overexpression promotes breast cancer development and chemoresistancebioRxivJune 2, 2023Yang Yu, Wen-Ming Cao, Feng Cheng, Zhongcheng Shi, Lili Han, Jin-Ling Yi, Edaise M da Silva, Higinio Dopeso, Hui Chen, Jianhua Yang, et al.sheared soluble chromatin. The soluble chromatin was precleared with Protein A Plus agarose beads (sc-2001, Santa Cruz Biotechnology) at 4 °C for one hour and then incubated with anti-FOXK2 (ab5298; Abcam) antibody

(8) Monitoring and assessment of lysosomal membrane damage in cultured cells using the high-content imagerSTAR ProtocolsJune 1, 2023Keisuke Tabata, Marika Saeki, Tamotsu Yoshimori, Maho Hamasaki,Santa Cruz Biotechnology,sc-23938 Mouse monoclonal anti-LAMP1 (H4A3) (1:500 dilution),Santa Cruz Biotechnology,sc-2001 Mouse monoclonal anti-LAMP2 (H4B4) (1:500 dilution),Santa Cruz Biotechnology,sc-18822 Alexa Fluor

(9) PTPA variants and impaired PP2A activity in early-onset parkinsonism with intellectual disabilityBrain : a journal of neurologyApril 19, 2023Fevga C, Tesson C, Carreras Mascaro A, Courtin T, van Coller R, Sakka S, Ferraro F, Farhat N, Bardien S, Damak M, et al.Protein lysates (100 µg) were mixed with 40 µl of protein A agarose slurry (Santa Cruz Biotechnology, sc-2001) and 2 µl of anti-PP2A C subunit antibody (Sigma-Aldrich, 05-421) in a total of 500 µl with pNPP Ser/Thr assay buffer

(10) Guanylate-binding protein 1 inhibits nuclear delivery of pseudorabies virus by disrupting structure of actin filamentsVeterinary researchMarch 14, 2023Zhang X, Du Q, Chen G, Jiang Y, Huang K, Li L, Tong D, Huang Y.staining solution (C1005) was purchased from Beyotime. Protein G-agarose (sc-2002) and protein A-agarose (sc-2001) were purchased from Santa Cruz. GTPase Kinetic ELIPA Assay Kit was purchased from Cytoskeleton (BK052). Quantitative PCR

(11) Water channel protein AQP1 in cytoplasm is a critical factor in breast cancer local invasionJournal of Experimental & Clinical Cancer ResearchFebruary 20, 2023Zhifang Guo, Huikun Zhang, Xiaoli Liu, Yawen Zhao, Yongzi Chen, Jiaqi Jin, Caixia Guo, Ming Zhang, Feng Gu, Yongjie Maovernight with indicated antibody or control IgG at 4 °C, on the second day, adding 40 µl protein A beads (sc-2001, Santa Cruz) into the protein-antibody complex overnight at 4 °C and washed to remove nonspecific binding.

(12) USP5-Beclin 1 axis overrides p53-dependent senescence and drives Kras-induced tumorigenicityNature communicationsDecember 17, 2022Li J, Wang Y, Luo Y, Liu Y, Yi Y, Li J, Pan Y, Li W, You W, Hu Q, et al.incubated with anti-Flag agarose beads or anti-Beclin 1 antibody followed by Protein A beads (Santa Cruz, sc-2001) to purify. After washing three times in binding buffer, proteins were eluted by mixing with loading

(13) BAF53A drives colorectal cancer development by regulating DUSP5-mediated ERK phosphorylationCell Death & DiseaseDecember 16, 2022Ziqing Yang, Dandan Huang, Manqi Meng, Wencong Wang, Junyan Feng, Lekun Fang, Honglei Chen, Shaomin Zouthe control IgG antibody, rotation at 4 °C overnight. Protein A/G agarose beads (50 μL, Santa Cruz, SC-2001) were then added to each sample. After 3 h, the beads were washed and followed by immunoblotting. For

(14) Activation of lysosomal mediated cell death in the course of autophagy by mTORC1 inhibitorScientific ReportsDecember 1, 2022Sameer Ullah Khan et al.(Santa Cruz, SC-6486), cathepsin L antibody (Santa Cruz, SC-6498), TFEB (H-125) SC-48784, LAMP1 (H4A3) SC-2001 Mouse monoclonal. Cell culture MIA PaCa-2 cells were obtained from the American Type Culture Collection

(15) Phenotype-based screening rediscovered benzopyran-embedded microtubule inhibitors as anti-neuroinflammatory agents by modulating the tubulin-p65 interactionExperimental & molecular medicineDecember 1, 2022Yim J, Lee J, Yi S, Koo JY, Oh S, Park H, Kim SS, Bae MA, Park J, Park SB.overnight. Subsequently, the proteins of interest were precipitated using protein A and G beads (Santa Cruz; sc-2001, sc-2002). Samples were analyzed with western blotting using anti-p65, IκB, and α-tubulin primary antibodies. Tubulin

(16) A seven-transmembrane protein-TM7SF3, resides in nuclear speckles and regulates alternative splicingiScienceNovember 18, 2022Roi Isaac, Yaron Vinik, Martin Mikl, Shani Nadav-Eliyahu, Hadas Shatz-Azoulay, Adi Yaakobi, Natalie DeForest, Amit R. Majithia, Nicholas J.G. Webster, Yaron Shav-Tal, et al.proteins","Chemicals, peptides, and recombinant proteins" ,, Protein A-agarose beads,Santa Cruz,Cat# sc-2001 Blocking peptide - IKELFQKEQPAGERTPL,Sigma,CustomizedN/A Actinomycin D,Sigma,Cat# A1410 High-capacity

(17) CdGAP maintains podocyte function and modulates focal adhesions in a Src kinase-dependent mannerSci RepNovember 4, 2022Jun Matsuda, Dina Greenberg, Sajida Ibrahim, Mirela Maier, Lamine Aoudjit, Jennifer Chapelle, Cindy Baldwin, Yi He, Nathalie Lamarche-Vane, Tomoko Takanoincubated with anti-GFP antibody overnight at 4 °C, and then with protein A agarose beads (Santa Cruz, sc-2001) for 1 h at 4 °C. Beads were washed three times, and proteins were eluted from the beads into SDS loading

(18) Ubiquitin proteolysis of a CDK-related kinase regulates titan cell formation and virulence in the fungal pathogen Cryptococcus neoformansNature communicationsOctober 27, 2022Cao C, Wang K, Wang Y, Liu TB, Rivera A, Xue C.with HA antibody (Genscript, A01244, 1:2000) and protein A agarose beads (Santa Cruz Biotechnology, sc-2001). After washing with kinase buffer (9802, Cell Signaling Technology) for 3 times, an aliquot of purified

(19) A COP1-GATA2 axis suppresses AR signaling and prostate cancerProceedings of the National Academy of SciencesOctober 25, 2022Tao Shen, Bingning Dong, Yanling Meng, David D. Moore, Feng Yang3-phosphate dehydrogenase (GAPDH; 6C5, sc-32233), Protein G PLUS-Agarose (sc-2002), and Protein A-Agarose (sc-2001) were purchased from Santa Cruz Biotechnology. Antibodies against Flag (M2, F3165), β-actin (A1978),

(20) RBCK1 is an endogenous inhibitor for triple negative breast cancer via hippo/YAP axisCell communication and signaling : CCSOctober 24, 2022Li Z, Su P, Ding Y, Gao H, Yang H, Li X, Yang X, Xia Y, Zhang C, Fu M, et al.cells were treated with 20 μM MG132 for 6 h. Then extract the overall protein and use 40 μL protein A (SC-2001, Santa Cruz) pre clarified the lysate for 4 h. The supernatant was collected and we use western blot

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Protein A-Agarose from Santa Cruz Biotechnology, Inc.

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Protein A-Agarose from Santa Cruz Biotechnology, Inc.

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